Prevalence of methicillin resistance in Staphylococcus pseudintermedius isolates from dogs with skin and ear infections in South Africa

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cg.authorship.typesCGIAR and developing country institute
cg.contributor.affiliationUniversity of Pretoria
cg.contributor.affiliationInternational Livestock Research Institute
cg.contributor.donorZoetis Animal Health
cg.coverage.countrySouth Africa
cg.coverage.iso3166-alpha2ZA
cg.coverage.regionAfrica
cg.coverage.regionSouthern Africa
cg.creator.identifierArshnee Moodley: 0000-0002-6469-3948
cg.howPublishedFormally Published
cg.identifier.doihttps://doi.org/10.36303/jsava.2022.93.1.505
cg.isijournalISI Journal
cg.issn1019-9128
cg.issue1
cg.journalJournal of the South African Veterinary Association
cg.reviewStatusPeer Review
cg.subject.ilriAMR
cg.subject.ilriANIMAL DISEASES
cg.subject.ilriANIMAL HEALTH
cg.subject.impactAreaNutrition, health and food security
cg.subject.sdgSDG 3 - Good health and well-being
cg.volume93
dc.contributor.authorPrior, C.D.
dc.contributor.authorMoodley, Arshnee
dc.contributor.authorKarama, M.
dc.contributor.authorMalahlela, M.N.
dc.contributor.authorLeisewitz, A.
dc.date.accessioned2022-08-15T12:28:31Zen
dc.date.available2022-08-15T12:28:31Zen
dc.identifier.urihttps://hdl.handle.net/10568/120536
dc.titlePrevalence of methicillin resistance in Staphylococcus pseudintermedius isolates from dogs with skin and ear infections in South Africaen
dcterms.abstractStaphylococcus pseudintermedius (SP) is an important opportunistic pathogen, frequently associated with pyoderma and otitis in dogs. The emergence and rapid expansion of methicillin-resistant Staphylococcus pseudintermedius (MRSP) is problematic due to multidrug resistance and reduced treatment options. The aim of this study was to determine i) the prevalence of MRSP in dogs with pyoderma or otitis externa, ii) the antimicrobial resistance patterns of MRSP from South African isolates, and iii) the risk factors for MRSP-associated pyoderma or otitis externa in dogs in South Africa (RSA). Sixty-eight presumptive clinical SP isolates (collected from 65 dogs) from five geographically dispersed laboratories in RSA were collected over 2 years. Possible MRSP isolates were flagged when resistance to oxacillin was observed. Thereafter, all isolates were confirmed as SP by polymerase chain reaction (PCR) and further genotyped for the mecA gene. Fifty-seven of 68 isolates were confirmed to be SP (83.8%), while 49/57 (85.9%) carried mecA. Our findings showed that preliminary phenotypic methods supplemented by genotypic methods increased the accuracy of correctly identifying SP. All isolates were resistant to at least one antimicrobial drug. There was a high incidence of amoxicillin (70.1%) and enrofloxacin (65%) resistance. Important risk factors for mecA positive carriage were previous hospital admission, pruritus, and previous antibacterial failure. This study demonstrates a high prevalence of mecA positive carriage (85.9% of samples) in MRSP pyoderma and otitis in dogs in RSA. There is an urgent need for better laboratory diagnosis of MRSP and surveillance of dogs presenting with pyoderma and otitis in South Africa.en
dcterms.accessRightsOpen Access
dcterms.audienceAcademics
dcterms.audienceScientists
dcterms.bibliographicCitationPrior, C.D., Moodley, A., Karama, M., Malahlela, M.N. and Leisewitz, A. 2022. Prevalence of methicillin resistance in Staphylococcus pseudintermedius isolates from dogs with skin and ear infections in South Africa. Journal of the South African Veterinary Association 93(1): 40a–40h.en
dcterms.extent40a-40h
dcterms.issued2022-06
dcterms.languageen
dcterms.licenseCC-BY-NC-3.0
dcterms.publisherMedpharm Publications
dcterms.subjectantimicrobial resistanceen
dcterms.subjectdogsen
dcterms.subjectanimal diseasesen
dcterms.subjectanimal healthen
dcterms.typeJournal Article

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