A reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay for detecting the pathogen of maize rough dwarf disease in China
| cg.coverage.country | China | |
| cg.coverage.iso3166-alpha2 | CN | |
| cg.identifier.doi | https://doi.org/10.1007/s13313-019-00649-9 | |
| cg.issn | 0815-3191 | |
| cg.issue | 5 | |
| cg.journal | Australasian Plant Pathology | |
| cg.volume | 48 | |
| dc.contributor.author | Du, Linlin | |
| dc.contributor.author | Shi, Wenjuan | |
| dc.contributor.author | Li, Xuejuan | |
| dc.contributor.author | Lan, Ying | |
| dc.contributor.author | Sun, Feng | |
| dc.contributor.author | Fan, Yongjian | |
| dc.contributor.author | Zhou, Tong | |
| dc.contributor.author | Zhou, Yijun | |
| dc.date.accessioned | 2024-12-19T12:54:09Z | en |
| dc.date.available | 2024-12-19T12:54:09Z | en |
| dc.identifier.uri | https://hdl.handle.net/10568/164669 | |
| dc.title | A reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay for detecting the pathogen of maize rough dwarf disease in China | en |
| dcterms.abstract | Maize rough dwarf disease (MRDD) is one of the most serious viral diseases of maize in China. The object of this study is to develop a reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay for rapid and sensitive detection of MRDD pathogen. Four RT-LAMP primers were designed on the basis of S10. Template RNA from infected leaves were used for RT-LAMP which were carried out under isothermal conditions at 61 °C for 90 min. RT-LAMP products were analyzed by electrophoresis in agarose gels or visual inspection of color change after staining with calcein. Sensitivity of the RT-LAMP assay was 100-fold higher than RT-PCR. This method can distinguish RBSDV from SRBSDV and the vectors carrying RBSDV from Rice stripe virus (RSV). The RT-LAMP assay shows excellent potential for application in the rapid and sensitive detection of MRDD pathogens in field samples or vector | en |
| dcterms.accessRights | Open Access | |
| dcterms.available | 2019-06-14 | |
| dcterms.bibliographicCitation | Du, Linlin; Shi, Wenjuan; Li, Xuejuan; Lan, Ying; Sun, Feng; Fan, Yongjian; Zhou, Tong and Zhou, Yijun. 2019. A reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay for detecting the pathogen of maize rough dwarf disease in China. Australasian Plant Pathol., Volume 48 no. 5 p. 485-489 | en |
| dcterms.extent | pp. 485-489 | |
| dcterms.issued | 2019-09 | |
| dcterms.language | en | |
| dcterms.license | CC-BY-4.0 | |
| dcterms.publisher | Springer | |
| dcterms.subject | plant science | en |
| dcterms.type | Journal Article |