Kinetics of interferon-gamma (IFN-gamma) release in the peripheral blood of calves vaccinated with BCG

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cg.identifier.doihttps://doi.org/10.1081/ias-120003664
cg.issn1532-1819
cg.issn1532-4230
cg.issue2
cg.journalJournal of Immunoassay and Immunochemistry
cg.subject.ilriCATTLE
cg.subject.ilriVACCINES
cg.subject.ilriBREEDS
cg.subject.ilriGENETICS
cg.subject.ilriLIVESTOCK
cg.volume23
dc.contributor.authorAmeni, G.
dc.contributor.authorTibbo, Markos
dc.date.accessioned2013-07-03T05:25:55Zen
dc.date.available2013-07-03T05:25:55Zen
dc.identifier.urihttps://hdl.handle.net/10568/33008
dc.titleKinetics of interferon-gamma (IFN-gamma) release in the peripheral blood of calves vaccinated with BCGen
dcterms.abstractA study was conducted on 13 Friesian-Zebu crossbred calves, the ages of which lie between 6 and 18 months, to investigate the kinetics of IFN-y release in the peripheral blood following Bacille Calmete Guerine (BCG) vaccination. After being screened for bovine tuberculosis (BTB), the calves were vaccinated with 1 m L inoculums containing 6 x 10 6 CFU of BCG. The level of IFN-y in the peripheral blood was measured two times before vaccination and seven times after vaccination, using a sandwich ELISA. The kinetics of IFN-y post vaccination presented itself in three phases: rising, falling, and steady phases. The concentration of IFN-y, before and after vaccination, both in stimulated and non-stimulated samples, was statistically significant (P < 0.01). Strong positive correlation (r-0.86) was recorded between the levels of IFN-y release in avian PPD- and bovine PPD-stimulated samples. Of the total 13 calves, 11 (84.6%) reacted positively to tuberculin inoculation 15 weeks post vaccination. It is concluded that the 1FN-y rises immediately after BCG vaccination, reaching its peak two weeks post vaccination, and then declines gradually in the following weeks. The strong positive reaction of calves to tuberculin inoculation 15 weeks post vaccination showed the capability of' BCG in causing the release of IFN-y in the peripheral blood, indicating its role in protection against infection with Mycobacterium bovis in calves.en
dcterms.accessRightsOpen Access
dcterms.bibliographicCitationJournal of Immunoassay & Immunochemistry;23(2): 245-251en
dcterms.extentp. 245-253
dcterms.issued2002-05-15
dcterms.languageen
dcterms.publisherInforma UK Limited
dcterms.subjectcalvesen
dcterms.subjectzebu cattleen
dcterms.subjectcrossbredsen
dcterms.subjectfriesian cattleen
dcterms.subjectvaccinationen
dcterms.subjecttuberculosisen
dcterms.subjectelisaen
dcterms.subjectblooden
dcterms.subjectmethodsen
dcterms.subjectbacillusen
dcterms.typeJournal Article

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