Loop-mediated isothermal amplification assay: A novel disease diagnostics tool in sweetpotato seed quality assurance

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cg.authorship.typesCGIAR and developing country institute
cg.authorship.typesCGIAR and advanced research institute
cg.contributor.affiliationKenya Agricultural and Livestock Research Organization
cg.contributor.affiliationInternational Potato Center
cg.contributor.donorBill & Melinda Gates Foundation
cg.coverage.regionSub-Saharan Africa
cg.creator.identifierBramwel Waswa Wanjala: 0000-0002-4732-1691
cg.creator.identifierJan Kreuze: 0000-0002-6116-9200
cg.creator.identifierMargaret A. McEwan: 0000-0001-8510-0526
cg.creator.identifierJan Low: 0000-0001-8170-6045
cg.howPublishedFormally Published
cg.identifier.doihttps://doi.org/10.1002/csc2.21203
cg.isijournalISI Journal
cg.issn1435-0653
cg.issue3
cg.journalCrop Science
cg.reviewStatusPeer Review
cg.subject.actionAreaResilient Agrifood Systems
cg.subject.actionAreaGenetic Innovation
cg.subject.cipSWEETPOTATOES
cg.subject.cipSWEETPOTATO AGRI-FOOD SYSTEMS
cg.subject.cipCROP PROTECTION
cg.subject.cipSEED SYSTEMS
cg.subject.cipFOOD SECURITY
cg.subject.cipCROP AND SEED SYSTEMS SCIENCES CSS
cg.subject.impactAreaNutrition, health and food security
cg.subject.sdgSDG 2 - Zero hunger
cg.subject.sdgSDG 13 - Climate action
cg.volume64
dc.contributor.authorWanjala, Bramwel W.
dc.contributor.authorKreuze, Jan F.
dc.contributor.authorMcEwan, M.
dc.date.accessioned2024-03-21T16:03:45Zen
dc.date.available2024-03-21T16:03:45Zen
dc.identifier.urihttps://hdl.handle.net/10568/140548
dc.titleLoop-mediated isothermal amplification assay: A novel disease diagnostics tool in sweetpotato seed quality assuranceen
dcterms.abstractSweetpotato is an important food crop in global production. However, sweetoptato viruses pose a threat to sustainable agriculture and cause significant economic loss. More than 30 viruses have been reported to date, with sweet potato feathery mottle virus (SPFMV) and sweet potato chlorotic stunt virus (SPCSV) occurring frequently and in combination, causing sweetpotato virus disease. The detection of viruses directly from sweetpotato is still a challenge, and efforts are being made toward this front. Therefore, rapid detection of viruses is critical for effective control. Current diagnostic tests are not sufficiently sensitive to reliably detect viruses directly from sweetpotato and require expensive laboratory equipment and a high level of skilled experience. Loop-mediated isothermal amplification (LAMP) is sensitive and specific for both DNA/RNA amplification; it is affordable and its characteristics make it potentially suitable for on-site testing. LAMP assays have been developed for several sweetpotato viruses: SPCSV, SPFMV, and sweet potato leaf curl virus (and related sweepoviruses). Laboratory validation showed a 100% diagnostic sensitivity for all three viruses. The LAMP on-site testing results were comparable to those of polymerase chain reaction and reverse transcriptase polymerase chain reaction confirmatory laboratory tests. Interlab validation and packaging into affordable kits will ensure high adoption in sub-Saharan Africa. The LAMP assay can be used for field surveys and monitoring the phytosanitary status of pre-basic seed production in quarantine or certification program. This guarantees pathogen-free planting material enters the seed system. We discuss opportunities for LAMP as a diagnostic assay for the rapid detection of sweetpotato viruses and its adoption as a quality control system for planting materials. Sweet potato feathery mottle virus (SPFMV), sweet potato chlorotic stunt virus (SPCSV), and sweet potato leaf curl virus (SPLCV) are the most common viruses known to cause disease in sweetpotato. Field deployable loop-mediated isothermal amplification (LAMP) assay for the rapid detection of SPFMV, SPCSV, and SPLCV. Adoption of the LAMP assay by field inspectors for field surveys and monitoring the phytosanitary status of pre-basic seed production in quarantine or certification programs.en
dcterms.accessRightsLimited Access
dcterms.audienceAcademics
dcterms.audienceCGIAR
dcterms.audienceDevelopment Practitioners
dcterms.audienceDonors
dcterms.audienceExtension
dcterms.audienceFarmers
dcterms.audienceGeneral Public
dcterms.audienceNGOs
dcterms.audiencePolicy Makers
dcterms.audienceScientists
dcterms.bibliographicCitationWanjala, B. W., Kreuze, J., McEwan, M., & Low, J. (2024). Loop‐mediated isothermal amplification assay: A novel disease diagnostics tool in sweetpotato seed quality assurance. Crop Science. ISSN 1435-0653. https://doi.org/10.1002/csc2.21203en
dcterms.extentpp. 1183-1192
dcterms.issued2024-02-15
dcterms.languageen
dcterms.licenseCopyrighted; all rights reserved
dcterms.publisherWiley
dcterms.subjectvirusen
dcterms.subjectCrinivirusen
dcterms.subjectyieldsen
dcterms.subjectpcren
dcterms.subjectsweet potatoesen
dcterms.subjectseed systemsen
dcterms.typeJournal Article

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