Phylogenetic analysis, mixed infection and seed transmission of Pea seed-borne mosaic virus in Ethiopia

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cg.contributor.affiliationInternational Center for Agricultural Research in the Dry Areas
cg.contributor.affiliationAmhara Regional Agricultural Research Institute
cg.contributor.affiliationAddis Ababa University
cg.contributor.affiliationAmhara Regional Agricultural Research Institute, Gondar Agricultural Research Center
cg.contributor.affiliationBotswana International University of Science & Technology
cg.contributor.donorCGIAR Trust Fund
cg.contributor.initiativePlant Health
cg.contributor.programAcceleratorSustainable Farming
cg.coverage.countryEthiopia
cg.coverage.iso3166-alpha2ET
cg.coverage.regionEastern Africa
cg.creator.identifierSafaa Kumari: 0000-0002-4492-6257
cg.creator.identifierAbdulrahman Moukahel: 0000-0003-4297-771X
cg.creator.identifierSeid Kemal: 0000-0002-1791-9369
cg.identifier.doihttps://doi.org/10.1016/j.pmpp.2024.102531
cg.isbn0885-5765
cg.isijournalISI Journal
cg.issn1096-1178
cg.journalPhysiological and Molecular Plant Pathology
cg.number102531
cg.reviewStatusPeer Review
cg.subject.actionAreaResilient Agrifood Systems
cg.subject.sdgSDG 1 - No poverty
cg.subject.sdgSDG 2 - Zero hunger
cg.subject.sdgSDG 15 - Life on land
cg.subject.sdgSDG 17 - Partnerships for the goals
cg.volume136
dc.contributor.authorAdeme, Anteneh
dc.contributor.authorKumari, Safaa
dc.contributor.authorMoukahel, Abdulrahman
dc.contributor.authorAlemu, Tesfaye
dc.contributor.authorAbraham, Adane
dc.contributor.authorAynewa, Yetsedaw
dc.contributor.authorGuadie, Demsachew
dc.contributor.authorKemal, Seid Ahmed
dc.date.accessioned2025-02-20T17:53:07Zen
dc.date.available2025-02-20T17:53:07Zen
dc.identifier.urihttps://hdl.handle.net/10568/173300
dc.titlePhylogenetic analysis, mixed infection and seed transmission of Pea seed-borne mosaic virus in Ethiopiaen
dcterms.abstractPea seed-borne mosaic virus (PSbMV) is a significant pathogen affecting the yield and quality of lentil and chickpea crops in Ethiopia's major legume-producing regions, Amhara and Oromia. This study focuses on characterizing PSbMV and its seed transmission rates, including mixed infections under field conditions. Tissue blot immunoassay (TBIA) analysis revealed that 56.2 % of tested lentil and chickpea samples were infected with PSbMV, often in mixed infections. PSbMV seed transmission rates varied widely among tested lentil genotypes, with the widely grown improved lentil cultivar Alemaya (FLIP 89-63 L) showing a transmission rate as high as 13.5 %. To characterize the isolates, the partial polyprotein gene was amplified and sequenced. Analysis of these sequences showed nucleotide identities ranging from 90.5 % to 100 %. Phylogenetic analysis classified Ethiopian PSbMV isolates (from lentil and chickpea) into four major clades and four subclades. Five lentil isolates (EthLe204-18, EthLe547-18, EthLe12-19, EthLe84-19, and EthLe214-33-19) and one chickpea isolate (EthCp115-19) clustered in subgroup BI, while isolate EthLe343-18 clustered into subgroup BII with an American pathotype-2 isolate (AJ252242). Isolates EthLe7-21 and EthLe381-21 clustered with an Australian P-2 isolate (HQ185579) into group D. Further analysis using RDP indicates intraspecific recombination only in isolate EthLe214-19, with fragments derived from EthLe343-18 and EthLe204-18 as major and minor parents, respectively. No recombination events were detected in the other isolates. This study suggests that variations in lentil genotype resistance to seed infection could be leveraged for resistance breeding, and the impacts of mixed infections on lentil crops warrant further investigation.en
dcterms.accessRightsLimited Access
dcterms.available2024-12-12
dcterms.bibliographicCitationAdeme, A. Kumari, S. G. Moukahel, A. Alemu, T. Abraham, A. Aynewa, Y. Guadie, D. and Ahmed, S. 2025. Phylogenetic analysis, mixed infection and seed transmission of Pea seed-borne mosaic virus in Ethiopia. Physiological and Molecular Plant Pathology, 136: 102531. https://doi.org/10.1016/j.pmpp.2024.102531en
dcterms.issued2025-01-16
dcterms.languageen
dcterms.publisherElsevier
dcterms.subjectphylogenetic analysisen
dcterms.subjectlentilen
dcterms.subjectchickpeaen
dcterms.subjectsequencingen
dcterms.subjecttbiaen
dcterms.subjectmixed infectionen
dcterms.subjectpsbmven
dcterms.typeJournal Article

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