Identification of a defective molecule derived from DNAA of the bipartite begomovirus of East African Cassava Mosaic Virus

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cg.authorship.typesCGIAR and developing country institute
cg.contributor.affiliationUniversity of Pretoria
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationInternational Laboratory for Tropical Agricultural Biotechnology
cg.contributor.affiliationAgricultural Research Council, South Africa
cg.contributor.affiliationUniversity of Greenwich
cg.contributor.donorDepartment for International Development, United Kingdom
cg.coverage.countrySouth Africa
cg.coverage.countryUganda
cg.coverage.countryUnited States
cg.coverage.countryUnited Kingdom
cg.coverage.iso3166-alpha2ZA
cg.coverage.iso3166-alpha2UG
cg.coverage.iso3166-alpha2US
cg.coverage.iso3166-alpha2GB
cg.coverage.regionAfrica
cg.coverage.regionACP
cg.coverage.regionSouthern Africa
cg.coverage.regionEastern Africa
cg.coverage.regionNorthern America
cg.coverage.regionEurope
cg.coverage.regionNorthern Europe
cg.identifier.doihttps://doi.org/10.1111/j.1365-3059.2005.01289.x
cg.isijournalISI Journal
cg.issn0032-0862
cg.issue1
cg.journalPlant Pathology
cg.reviewStatusPeer Review
cg.subject.iitaPLANT DISEASES
cg.subject.iitaPESTS OF PLANTS
cg.subject.iitaPLANT GENETIC RESOURCES
cg.subject.iitaPLANT BREEDING
cg.subject.iitaCASSAVA
cg.volume55
dc.contributor.authorNdunguru, J.
dc.contributor.authorLegg, James P.
dc.contributor.authorFofana, B.
dc.contributor.authorAveling, T.
dc.contributor.authorThompson, G.
dc.contributor.authorFauquet, Claude M.
dc.date.accessioned2019-03-03T05:53:34Zen
dc.date.available2019-03-03T05:53:34Zen
dc.identifier.urihttps://hdl.handle.net/10568/99850
dc.titleIdentification of a defective molecule derived from DNAA of the bipartite begomovirus of East African Cassava Mosaic Virusen
dcterms.abstractGeminivirus defective interfering DNAs arise spontaneously in mechanically inoculated test plants, and have previously been found with DNA‐B of the bipartite cassava mosaic geminiviruses, but not DNA‐A. Reported here for the first time is the cloning and characterization of a naturally occurring truncated form of cassava mosaic geminivirus DNA‐A, which at 1525 nt is around half the expected full size. Sequence analysis has shown it to be a defective (df) form of East African cassava mosaic virus (EACMV) DNA‐A that has retained its cis elements essential for replication by the helper virus, and it has been termed df DNA‐A 15. Phylogenetic comparisons placed the df DNA‐A 15 molecule close to mild and severe isolates of EACMV‐UG2. Biolistic inoculation of Nicotiana benthamiana with infectious df DNA‐A 15 clone and East African cassava mosaic Cameroon virus (EACMCV) resulted in symptom amelioration as compared with EACMCV singly inoculated plants, and there was an accumulation of df DNA‐A 15 in systemically infected leaves. In addition, the level of EACMV DNA‐B accumulation was reduced in the coinoculated plants compared with those inoculated with EACMCV alone. PCR and sequence analysis confirmed the helper virus as EACMV.en
dcterms.accessRightsLimited Access
dcterms.available2005-10-14
dcterms.bibliographicCitationNdunguru, J., Legg, J., Fofana, B., Aveling, T., Thompson, G. & Fauquet, C. (2006). Identification of a defective molecule derived from DNA-A of the bipartite begomovirus of East African Cassava Mosaic Virus. Plant Pathology, 55(1), 2-10.en
dcterms.extentp. 2-10
dcterms.issued2006-02
dcterms.languageen
dcterms.licenseCopyrighted; all rights reserved
dcterms.publisherWiley
dcterms.subjectcassava mosaic virusen
dcterms.subjectafrican cassava mosaic virusen
dcterms.subjectcassavaen
dcterms.subjecthorticultureen
dcterms.subjectgeneticsen
dcterms.typeJournal Article

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